RT-PCR

Thermo Scientific offers tools for both continuous (one-step) and = uncoupled=20 (two-step) RT-PCR

From: Subject: Finnzymes Reagents - Products - RT-PCR Date: Wed, 17 Aug 2011 18:38:17 +0530 MIME-Version: 1.0 Content-Type: multipart/related; type="text/html"; boundary="----=_NextPart_000_026C_01CC5D0C.D49420D0" X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6109 This is a multi-part message in MIME format. ------=_NextPart_000_026C_01CC5D0C.D49420D0 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Content-Location: http://www.finnzymes.com/rt-pcr/rt-pcr.html Finnzymes Reagents - Products - RT-PCR

Part of Thermo Fisher Scientific

Reverse Transcription PCR (RT-PCR) couples reverse transcription (RT) = of RNA=20 with amplification of the resulting cDNA by PCR. RT-PCR is a rapid and = sensitive=20 method for analyzing gene expression, for determining the presence or = absence of=20 transcripts and for producing cDNA for cloning.

Instruments

Consumables

RT-PCR Kits

Two-step kit: = Phusion=AE RT-PCR=20 Kit
One-step kits: = RobusT=99 RT-PCR=20 Kits

Stand-alone enzymes

A= MV=20 Reverse Transcriptase
M-MuLV=20 Reverse Transcriptase, RNase H^-

cDNA synthesis for quantitative PCR

DyNAmo=99=20 cDNA synthesis kit

FAQ =96 RT-PCR = Techni= cal=20 Appendix =96 RT-PCR RT-PCR = References=20

Ratastie 2, 01620 Vantaa, Finland = -=20 Tel. +358 9 329 100 - Fax +358 9 3291=20 0500

Learn more about other Thermo Scientific Life Science = Research=20 Products

Biopolymers

Nucleic acid technologies

Cell Culture

Protein Research

Antibodies

PCR Reagents and Plastics

High Content Screening

RNAi Technologies

Gene Expression

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this.op5=3D(this.agent.indexOf("opera 5")>-1 || = this.agent.indexOf("opera/5")>-1) && window.opera =0A= this.op6=3D(this.agent.indexOf("opera 6")>-1 || = this.agent.indexOf("opera/6")>-1) && window.opera =0A= this.ie5 =3D (this.agent.indexOf("msie 5")>-1 && !this.op5 && = !this.op6)=0A= this.ie55 =3D (this.ie5 && this.agent.indexOf("msie 5.5")>-1)=0A= this.ie6 =3D (this.agent.indexOf("msie 6")>-1 && !this.op5 && = !this.op6)=0A= this.ie4=3D(this.agent.indexOf("msie")>-1 && document.all &&!this.op5 = &&!this.op6 &&!this.ie5&&!this.ie6)=0A= this.ie =3D (this.ie4 || this.ie5 || this.ie6)=0A= this.mac=3D(this.agent.indexOf("mac")>-1)=0A= this.ns6=3D(this.agent.indexOf("gecko")>-1 || window.sidebar)=0A= this.ns4=3D(!this.dom && document.layers)?1:0;=0A= this.bw=3D(this.ie6 || this.ie5 || this.ie4 || this.ns4 || this.ns6 || = this.op5 || this.op6)=0A= this.usedom=3D this.ns6//Use dom creation=0A= this.reuse =3D this.ie||this.usedom //Reuse layers=0A= this.px=3Dthis.dom&&!this.op5?"px":""=0A= return this=0A= }=0A= var bw=3Dnew cm_bwcheck()=0A= /*Variable declaration*/=0A= var cmpage=0A= /*Crossbrowser objects functions*/=0A= function cm_message(txt){alert(txt); return false}=0A= function cm_makeObj(obj,nest,o,doc){ //Changed in v4.05=0A= if(!doc) doc=3Ddocument =0A= if(bw.usedom&&o) this.evnt=3Do=0A= else{nest=3D(!nest) ? "doc.":'doc.layers.'+nest+'.'=0A= this.evnt=3Dbw.dom? doc.getElementById(obj):=0A= bw.ie4?doc.all[obj]:bw.ns4?eval(nest+"layers." +obj):0;=0A= }=0A= if(!this.evnt) return cm_message('The layer does not exist ('+obj+')' =0A= +'- \nIf your using Netscape please check the nesting of your tags = (on the entire page)\nNest:'+nest)=0A= this.css=3Dbw.dom||bw.ie4?this.evnt.style:this.evnt; this.ok=3D0=0A= this.ref=3Dbw.dom||bw.ie4?doc:this.css.document; =0A= this.obj =3D obj + "Object"; eval(this.obj + "=3Dthis");=0A= this.x=3D0; this.y=3D0; this.w=3D0; this.h=3D0; this.vis=3D0; return = this=0A= }=0A= cm_makeObj.prototype.moveIt =3D function(x,y){this.x=3Dx;this.y=3Dy; = this.css.left=3Dx+bw.px;this.css.top=3Dy+bw.px}=0A= cm_makeObj.prototype.showIt =3D = function(o){this.css.visibility=3D"visible"; this.vis=3D1; = if(bw.op5&&this.arr){ this.arr.showIt(); }}//alert('showing arrow')}} =0A= cm_makeObj.prototype.hideIt =3D = function(no){this.css.visibility=3D"hidden"; this.vis=3D0;}=0A= cm_makeObj.prototype.clipTo =3D function(t,r,b,l,setwidth){ =0A= this.w=3Dr; this.h=3Db; = if(bw.ns4){this.css.clip.top=3Dt;this.css.clip.right=3Dr; = this.css.clip.bottom=3Db;this.css.clip.left=3Dl=0A= }else{if(t<0)t=3D0;if(r<0)r=3D0;if(b<0)b=3D0;if(b<0)b=3D0; = this.css.clip=3D"rect("+t+bw.px+","+r+bw.px+","+b+bw.px+","+l+bw.px+")";=0A= if(setwidth){if(bw.op5||bw.op6){this.css.pixelWidth=3Dr; = this.css.pixelHeight=3Db;}else{this.css.width=3Dr+bw.px; = this.css.height=3Db+bw.px;}}}}=0A= function cm_active(on,h){=0A= if(this.o.arr) = on?this.o.arr.hideIt():bw.op5?this.o.arr.showIt():this.o.arr.css.visibili= ty=3D"inherit"=0A= if(bw.reuse||bw.usedom){=0A= if(!this.img2) this.o.evnt.className=3Don?this.cl2:this.cl=0A= else = this.o.ref.images["img"+this.name].src=3Don?this.img2.src:this.img1.src; = //Changed v4.05=0A= if(on && bw.ns6){this.o.hideIt(); this.o.css.visibility=3D'inherit' = }; //netscape 6 bug fix =0A= }else{ =0A= if(!this.img2){ if(on) this.o.over.showIt(); else = this.o.over.hideIt();=0A= }else = this.o.ref.images["img"+this.name].src=3Don?this.img2.src:this.img1.src;=0A= }this.isactive=3Don?1:0=0A= }=0A= /***Pageobject **/=0A= function cm_page(frame){ //Changed v4.05=0A= if(!frame) frame =3D self =0A= this.x=3D0; this.x2 = =3D(!bw.ie)?frame.innerWidth:frame.document.body.offsetWidth-20;=0A= this.y=3D0; this.orgy=3Dthis.y2=3D = (!bw.ie)?frame.innerHeight:frame.document.body.offsetHeight-6;=0A= this.x50=3Dthis.x2/2; this.y50=3Dthis.y2/2; return this=0A= }=0A= /***check positions**/=0A= function cm_cp(num,w,minus){=0A= if(num){if(num.toString().indexOf("%")!=3D-1){var t =3D = w?cmpage.x2:cmpage.y2; num=3DparseInt((t*parseFloat(num)/100))=0A= if(minus) num-=3Dminus }else num=3Deval(num);} else num=3D0; return num=0A= }=0A= /**Level object**/=0A= function cm_makeLevel(){=0A= var c=3Dthis, a=3Darguments; c.width=3Da[0]||null; = c.height=3Da[1]||null; =0A= c.regClass=3Da[2]||null; c.overClass=3Da[3]||null; = c.borderX=3Da[4]||null; =0A= c.borderY=3Da[5]||null; c.borderClass=3Da[6]||null; = c.rows=3Da[7]>-1?a[7]:null; =0A= c.align=3Da[8]||null; c.offsetX=3Da[9]||null; c.offsetY=3Da[10]||null; = c.arrow=3Da[11]||null; =0A= c.arrowWidth=3Da[12]||null; c.arrowHeight=3Da[13]||null; return c=0A= }=0A= /***Making the main menu object**/=0A= function makeCM(name){ //Changed v4.05=0A= var c=3Dthis; c.mc=3D0; c.name =3D name; c.m=3Dnew Array(); = c.level=3Dnew Array(); c.l=3Dnew Array(); c.tim=3D100; c.isresized=3D0;=0A= c.isover=3D0; c.zIndex=3D100; c.frameStartLevel=3D1; c.bar=3D0; = c.z=3D0; c.totw=3D0; c.toth=3D0; c.maxw=3D0; c.maxh=3D0; cmpage =3D new = cm_page(); c.constructed =3D 0;=0A= return this=0A= }//events=0A= makeCM.prototype.onshow=3D""; makeCM.prototype.onhide=3D""; = makeCM.prototype.onconstruct=3D"";=0A= /***Creating layers**/=0A= function cm_divCreate(id,cl,txt,w,c,app,ex,txt2){=0A= if(bw.usedom){var div=3Ddocument.createElement("DIV"); = div.className=3Dcl; div.id=3Did; =0A= if(txt) div.innerHTML=3Dtxt; if(app){app.appendChild(div); return = div}=0A= if(w) document.body.appendChild(div); return div=0A= }else{var dstr=3D'

"+txt; ; if(txt2) = dstr+=3Dtxt2; =0A= if(c) dstr+=3D'

'; if(w) document.write(dstr); else return dstr=0A= }return ""=0A= }=0A= /***Getting layer string for each menu**/=0A= function cm_getLayerStr(m,app,name,fill,clb,arrow,ah,aw,root){=0A= var no=3Dm.nolink,arrstr=3D'',l=3Dm.lev,str=3D'',txt=3Dm.txt,ev=3D'', = id=3Dname + '_' + m.name,d1; if(app) d1=3Dapp=0A= if((!bw.reuse||l=3D=3D0) && !no){=0A= ev=3D' onmouseover=3D"'+name+'.showsub(\''+m.name+'\')"' =0A= +' onmouseout=3D"'+name+'.mout(\''+m.name+'\')"'=0A= +' onclick=3D"'+name+'.onclck(\''+m.name+'\'); return false" '=0A= }=0A= if(bw.reuse&&l!=3D0) txt=3D''; if(l=3D=3D0) = str+=3Dd1=3Dcm_divCreate(id+'_0',clb,''); = str+=3Dm.d2=3Dcm_divCreate(id,m.cl,txt,0,0,d1,ev)=0A= if(l=3D=3D0&&bw.usedom){ =0A= m.d2.onclick=3Dnew Function(name+'.onclck("'+m.name+'")'); =0A= m.d1=3Dd1; =0A= m.d2.onmouseover=3Dnew Function(name+'.showsub("'+m.name+'")'); =0A= m.d2.onmouseout=3Dnew Function(name+'.mout("'+m.name+'")')=0A= }if(!bw.reuse && !m.img1 && !no){=0A= str+=3Dcm_divCreate(id+'_1',m.cl2,txt,0,1)=0A= str+=3Dcm_divCreate(id+'_3',"clCMAbs",'

',0,1)=0A= }str+=3D''; =0A= = if(l=3D=3D0){if(arrow)str+=3Dm.d3=3Dcm_divCreate(id+'_a','clCMAbs','

',0,1,d1); str+=3D""}=0A= str+=3D"\n"; if(!bw.reuse){m.txt=3Dnull; m.d2=3Dnull; m.d3=3Dnull;}=0A= if(bw.usedom){ if(l=3D=3D0) document.body.appendChild(d1); str=3D''}=0A= return str=0A= }=0A= /***get align num from text (better to evaluate numbers later)**/=0A= function cm_checkalign(a){=0A= switch(a){=0A= case "right": return 1; break; case "left": return 2; break;=0A= case "bottom": return 3; break; case "top": return 4; break;=0A= case "righttop": return 5; break; case "lefttop": return 6; break;=0A= case "bottomleft": return 7; break; case "topleft": return 8; break;=0A= }return null=0A= }=0A= /**Making each individual menu **/=0A= makeCM.prototype.makeMenu=3Dfunction(name,parent,txt,lnk,targ,w,h,img1,im= g2,cl,cl2,align,rows,nolink,onclick,onmouseover,onmouseout){=0A= var c =3D this; if(!name) name =3D c.name+""+c.mc; var p =3D = parent!=3D""&&parent&&c.m[parent]?parent:0;=0A= if(c.mc=3D=3D0){var tmp=3Dlocation.href;=0A= if(tmp.indexOf('file:')>-1||tmp.charAt(1)=3D=3D':') = c.root=3Dc.offlineRoot; else c.root=3Dc.onlineRoot=0A= if(c.useBar){if(!c.barBorderClass) c.barBorderClass=3Dc.barClass; = c.bar1 =3D cm_divCreate(c.name+'bbar_0',c.barClass,'',0,1);=0A= c.bar =3D = cm_divCreate(c.name+'bbar',c.barBorderClass,'',1,1,0,0,c.bar1); = if(bw.usedom) c.bar.appendChild(c.bar1); =0A= }}var create=3D1,img,arrow; var m =3D c.m[name] =3D new Object(); = m.name=3Dname; m.subs=3Dnew Array(); m.parent=3Dp; m.arnum=3D0; m.arr=3D0=0A= var l =3D m.lev =3D p?c.m[p].lev+1:0; c.mc++; m.hide=3D0;=0A= if(l>=3Dc.l.length){=0A= var p1,p2=3D0; if(l>=3Dc.level.length) p1=3Dc.l[c.level.length-1];=0A= else p1=3Dc.level[l]; c.l[l]=3Dnew Array(); if(!p2) p2=3Dc.l[l-1]=0A= if(l!=3D0){ if(isNaN(p1.align)) p1["align"]=3Dcm_checkalign(p1.align)=0A= for(i in p1){if(i!=3D"str"&&i!=3D"m"){if(p1[i]=3D=3Dnull) = c.l[l][i]=3Dp2[i]; else c.l[l][i]=3Dp1[i] }}=0A= }else{c.l[l]=3Dc.level[0]; = c.l[l].align=3Dcm_checkalign(c.l[l].align)}=0A= c.l[l]["str"]=3D''; c.l[l].m=3Dnew Array(); if(!c.l[l].borderClass) = c.l[l].borderClass=3Dc.l[l].regClass=0A= c.l[l].app=3D0; c.l[l].max=3D0; c.l[l].arnum=3D0; c.l[l].o=3Dnew = Array(); c.l[l].arr=3Dnew Array()=0A= c.level[l]=3Dp1=3Dp2=3Dnull=0A= if(l!=3D0) c.l[l].str=3Dc.l[l].app=3Dcm_divCreate(c.name+ '_' = +l+'_0',c.l[l].borderClass,'')=0A= }if(p){p =3D c.m[p]; p.subs[p.subs.length]=3Dname; =0A= if(p.subs.length=3D=3D1&&c.l[l-1].arrow){ p.arr=3D1; =0A= if(p.parent){c.m[p.parent].arnum++=0A= if(c.m[p.parent].arnum>c.l[l-1].arnum){=0A= = c.l[l-1].str+=3Dc.l[l-1].arr[c.l[l-1].arnum]=3Dcm_divCreate(c.name+ '_a' = +(l-1)+'_'+c.l[l-1].arnum,'clCMAbs','

',0,1,c.l[l-1].app); = c.l[l-1].arnum++=0A= }}}if(bw.reuse) if(p.subs.length>c.l[l].max) c.l[l].max =3D = p.subs.length; else create=3D0=0A= }m.rows=3Drows>-1?rows:c.l[l].rows; m.w=3Dcm_cp(w||c.l[l].width,1); = m.h=3Dcm_cp(h||c.l[l].height,0); m.txt=3Dtxt; m.lnk=3Dlnk; =0A= if(align) align=3Dcm_checkalign(align); m.align=3Dalign||c.l[l].align; = m.cl=3Dcl=3Dcl||c.l[l].regClass; =0A= m.targ=3Dtarg; m.cl2=3Dcl2||c.l[l].overClass; m.create=3Dcreate; = m.mover=3Donmouseover; m.out=3Donmouseout; =0A= m.onclck=3Donclick; m.active =3D cm_active; m.isactive=3D0; = m.nolink=3Dnolink=0A= if(create) c.l[l].m[c.l[l].m.length]=3Dname=0A= if(img1){m.img1 =3D new Image(); m.img1.src=3Dc.root+img1; if(!img2) = img2=3Dimg1; m.img2 =3D new Image(); m.img2.src=3Dc.root+img2;=0A= m.cl=3D"clCMAbs"; m.txt=3D''; if(!bw.reuse&&!nolink) m.txt =3D '';;=0A= m.txt+=3D' 3D""

'; if(!bw.reuse&&!nolink) m.txt+=3D''=0A= }else{m.img1=3D0; m.img2=3D0}; =0A= if(l=3D=3D0||create) = c.l[l].str+=3Dcm_getLayerStr(m,c.l[l].app,c.name,c.fillImg,c.l[l].borderC= lass,c.l[l].arrow,c.l[l].arrowWidth,c.l[l].arrowHeight,c.root)=0A= if(l=3D=3D0){if(m.w>c.maxw) c.maxw=3Dm.w; if(m.h>c.maxh) c.maxh=3Dm.h; = c.totw+=3Dc.pxBetween+m.w+c.l[0].borderX;c.toth+=3Dc.pxBetween+m.h+c.l[0]= .borderY}=0A= if(lnk && !onmouseover) m.mover=3D"self.status=3D'"+c.root+m.lnk+"'"=0A= }=0A= /**Getting x/y coords for subs **/=0A= makeCM.prototype.getcoords=3Dfunction(m,bx,by,x,y,maxw,maxh,ox,oy){=0A= var a=3Dm.align; x+=3Dm.o.x; y+=3Dm.o.y=0A= switch(a){=0A= case 1: x+=3Dm.w+bx; break; case 2: x-=3Dmaxw+bx; break;=0A= case 3: y+=3Dm.h+by; break; case 4: y-=3Dmaxh+by; break;=0A= case 5: x-=3Dmaxw+bx; y-=3Dmaxh-m.h; break;=0A= case 6: x+=3Dm.w+bx; y-=3Dmaxh-m.h; break;=0A= case 7: y+=3Dm.h+by; x-=3Dmaxw-m.w; break;=0A= case 8: y-=3Dmaxh+by; x-=3Dmaxw-m.w+bx; break;=0A= }//Added v4.05=0A= if(m.lev=3D=3Dthis.frameStartLevel-1 && this.frames){=0A= switch(a){=0A= case 1: x=3D0; break; =0A= case 2: x=3Dthis.cmpage.x2-maxw; break;=0A= case 3: y=3D0; break; =0A= case 4: y-=3Dmaxh+by; break;=0A= case 5: x-=3Dmaxw+bx; y-=3Dmaxh-m.h; break;=0A= case 6: x+=3Dm.w+bx; y-=3Dmaxh-m.h; break;=0A= case 7: y+=3Dm.h+by; x-=3Dmaxw-m.w; break;=0A= case 8: y-=3Dmaxh+by; x-=3Dmaxw-m.w+bx; break;=0A= }=0A= }=0A= m.subx=3Dx + ox; m.suby=3Dy + oy=0A= }=0A= /**Showing sub elements**/=0A= makeCM.prototype.showsub=3Dfunction(el){ //Changed v4.05=0A= var c=3Dthis,pm=3Dc.m[el];=0A= if(!pm.b||(c.isresized&&pm.lev>0)) pm.b=3Dc.l[pm.lev].b; c.isover=3D1=0A= clearTimeout(c.tim);=0A= var ln=3Dpm.subs.length,l=3Dpm.lev+1=0A= if(c.l[pm.lev].a=3D=3Del&&l!=3Dc.l.length){if(c.l[pm.lev+1].a) = c.hidesub(l+1,el); return}=0A= c.hidesub(l,el); if(pm.mover) eval(pm.mover); if(!pm.isactive) = pm.active(1);=0A= c.l[pm.lev].a =3D el; if(ln=3D=3D0) return; =0A= if(!c.l[l].b) return //Added v4.05=0A= var b =3D c.l[l].b, bx=3Dc.l[l].borderX, by=3Dc.l[l].borderY, = rows=3Dpm.rows=0A= var x=3Dbx,y=3Dby,maxw=3D0,maxh=3D0,cn=3D0; b.hideIt()=0A= for(var i=3D0;imaxw) maxw=3Dm.w; maxh=3Dy}=0A= else{x+=3Dm.w+bx; if(m.h>maxh) maxh=3Dm.h; maxw=3Dx;}=0A= o.css.visibility=3D"inherit"; if(bw.op5||bw.op6) o.showIt()=0A= }else{o =3D c.m[c.l[l].m[i]].o; o.hideIt();} }=0A= if(!rows) maxw+=3Dbx*2; else maxh+=3Dby*2; b.clipTo(0,maxw,maxh,0,1)=0A= //Check frame scroll=0A= if(c.chkscroll) c.chkscroll() //Added v4.05 - not the best solution=0A= if(!pm.subx||!pm.suby||c.scrollY>0||c.isresized) = c.getcoords(pm,c.l[l-1].borderX,c.l[l-1].borderY,pm.b.x,pm.b.y,maxw,maxh,= c.l[l-1].offsetX,c.l[l-1].offsetY)=0A= x=3Dpm.subx; y=3Dpm.suby; if(c.chkscroll&&l>=3Dc.frameStartLevel) = y+=3Dc.scrollY; b.moveIt(x,y); if(c.onshow) eval(c.onshow); b.showIt()=0A= }=0A= /**Hide sub elements **/=0A= makeCM.prototype.hidesub=3Dfunction(l,el){ //Changed v4.05=0A= var c =3D this,tmp,m,i,j,hide=0A= if(!l) {l=3D1; hide=3D1}=0A= 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